Conversion of cyclamate to cyclohexylamine in guinea pig.

Continuous oral administration of sodium cyclamate(CHS-Na) to guinea pig caused the gradual increase of urinary excretion of cyclohexylamine(CHA) after a certain period. The amount of CHA excreted was not only different in individuals, but also dependent on each experiment. It was diminished when CHS-Na was administered orally with antibiotics or intraperitoneally. It was shown from the incubation experiments that apparent accumulation of CHA was observed by cecal contents or feces from the animal excreting CHA in urine by oral administration of CHS-Na, but not from that diminished the excretion by the above conditions. Any in vitro study using animal tissues failed to detect the accumulation of CHA. Consequently it may be probable to assume the participation of duct microflora in the conversion of CHS-Na to CHA in guinea pig.